This work presents a comparative study of the miRNA transcriptome of mature and developing B. The predicted miRNA target genes encode a broad range of proteins related to seed development and energy storage. Analysis of miRNA population between libraries reveals that several miRNAs and isomiRNAs have different abundance in developing stages compared to mature seeds. Assembled mRNA-seq contigs allowed for a search of putative new precursors and led to the identification of 13 novel miRNA families. napus but conserved in other plant species were identified by alignment with known plant mature miRNAs.
napus additionally 29 families not reported in B. Among these miRNA families, 17 families are currently known to exist in B.
Members of 59 miRNA families were detected through a computational analysis of a large number of reads obtained from deep sequencing two small RNA and two RNA-seq libraries of (i) pooled immature developing stages and (ii) mature B. napus by identifying plant-conserved and novel miRNAs and comparing miRNA abundance in mature versus developing seeds. This work aims to describe the miRNAome of developing seeds of B. napus is currently poorly characterized, especially at different seed developmental stages. In Brassica napus, an important edible oil crop, valuable lipids are synthesized and stored in specific seed tissues during embryogenesis. MicroRNAs (miRNAs) are important post-transcriptional regulators of plant development and seed formation.
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